Genome Lytic phage pPM_01 Proteus mirabilis Urinary tract infection. The inability to lysogenize a host, the absence of endotoxin genes in the annotated genome, and the lytic behavior suggest phage pPM_01 as a possible safe biological candidate to control P. Host range determination and SDS-PAGE analysis were also performed.

The whole genome sequence showed a low similarity to known phages (i.e., Enterobacter phage Enc34 and Enterobacteria phage Chi). Reads were paired using вЂSet paired readsвЂ™ with an expected insert size of 500 bp. indicum paired-end fastq dataset was imported into Geneious Prime as two lists. Functional clusters of related potential genes were defined (structural, lytic, packaging, replication, modification, and modulatory). read data were generated by Illumina-Solexa GAIIx, see Zhang et. Seventy putative genes were identified and annotated using various bioinformatics tools including RAST, Geneious v.R7, National Center for Biotechnology Information (NCBI) BLAST, and tRNAscan-SE-v1.3 Search. The whole genome contains a total of 58,546 bp of linear double-stranded DNA with a G+C content of 46.9%. The genome was sequenced using Illumina technology and the reads obtained were assembled using CLC Genomic Workbench v.7.0.3. mirabilis), with its complete genome sequence successfully determined. Researchers can easily query, manage, share, and analyze all of their sequence data intuitively within a user-friendly interface. Transmission electron microscopy revealed phage pPM_01 to be a siphovirus (the first reported virus to infect P. Geneious R10 provides a solution to these problems by incorporating industry-leading algorithms and applications for genomic & protein sequence analyses via a single robust desktop program. In this paper, we characterize the biology and complete genome sequence of the phage. CRITICAL STEP: All bacterial cultures should be treated as potentially pathogenic to the laboratory worker and colleagues. Phage pPM_01 was previously isolated from a raw sewage treatment facility located in Batu Maung, Penang, Malaysia, and it was highly lytic against Proteus mirabilis, which causes urinary tract infections in humans. Pellet the liquid culture media (200 l) by centrifuging at 8000 g for 8 min in a sterile microfuge tube.

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