However, previous research suggested that the nutrient availability of DDGS in monogastric animals is limited due to the high concentration of non-starch polysaccharides (NSPs, also known as indigestible fibre), which comprise 25вЂ“33% of DDGS. The use of вЂњcorn distillers dried grains with solublesвЂќ (DDGS) in animal feed has recently received extensive attention because of its good nutritional profile including protein, fat, vitamins, and minerals. Non-starch polysaccharide, feed enzyme, Paenibacillus, endo-1, 4-ОІ-xylanase They showed high solubilizing activities toward a feed material, corn dried distillerвЂ™s grains with solubles, compared to five previously characterized xylanases.ДЄbbreviations : CX: corn fibre xylan DDGS: corn dried distillerвЂ™s grains with solubles Their activities toward beechwood xylan and/or wheat arabinoxylan indicated that these enzymes are ОІ-1,4-xylanases. Phylogenetic analysis classified Xyn5A in subfamily 35 of GH family 5, a subfamily of unknown function. Four enzymes, Xyn5A, Xyn10B, Xyn11A, and Xyn30A, were successfully identified, which belong to glycoside hydrolase (GH) families, 5, 10, 11, and 30, respectively.

Enzymes were purified from supernatant of culture broth of strain H2C based on solubilizing activities toward CX.

Analysis based on the 16S rRNA sequence placed strain H2C within genus Paenibacillus. To find enzymes capable of hydrolyzing CX, we isolated a bacterial strain (named H2C) from soil, by enrichment culture using non-starch polysaccharides of corn as the sole carbon source. Corn fibre xylan (CX) shows high resistance to enzymatic hydrolysis due to its densely decorated side chains.

Histalon xtractor buffer